Description
| C-TOM-CE Phosphoramidite |
Catalog Number: 10-3014-xx
Description: C-TOM-CE Phosphoramidite
| 5"-Dimethoxytrityl-N-acetyl-Cytidine,2"-O-triisopropylsilyloxymethyl- 3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C51H72N5O10PSi | M.W.: 974.22 | F.W.: 305.18 |
Diluent: Anhydrous Acetonitrile |
| Coupling: See Technical Bulletin |
| Deprotection: See Technical Bulletin for details (www.glenresearch.com/Technical/TB_RNA_TOM_Deprotection.pdf). Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 24 hours |
| Please Note: TOM-RNA Phosphoramidites are supplied under agreement with QIAGEN.RNA synthesis using the TOM-Protecting-Group is covered by US Patent No. 5,986,084. |
TOM-Protected RNA Phosphoramidites
RNA synthesis using monomers containing the2’-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) ischaracterized by very high coupling efficiency along with fast, simpledeprotection. High coupling efficiency is achieved because theTOM-Protecting-Group exhibits lower steric hindrance than the2’-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers.Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature.A further feature of the TOM-Protecting-Group is that during basic steps it cannot undergo 2’ to 3’ migration. This migration under basic conditions leads tonon-biologically active 2’-5’ linkages when using the TBDMS group. Thesefeatures allow the TOM-Protected monomers to produce longer oligonucleotides.TOM-Protected RNA monomers are also fully compatible with minor bases with2’-O-TBDMS protection.
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