Cambio/5-Ethynyl-dU-CE Phosphoramidite/10-1554-95-免疫在线蚂蚁淘旗下平台

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Cambio/5-Ethynyl-dU-CE Phosphoramidite/10-1554-95

商品编号： 10-1554-95

品牌： Cambio

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产地：美国(厂家直采)

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产品分类：其他

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电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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Description

5-Ethynyl-dU offers convenient click conjugation with an azide to generate a label rigidly attached to one of the oligonucleotide bases. 5-Ethynyl-dU is subject to base-catalyzed hydration during cleavage and deprotection, especially when using a strong base or heat. Hydration of an ethynyl group forms a methyl ketone which subsequently blocks potential click reactions. Mild deprotection conditions are necessary when using 5-Ethynyl-dU-CE Phosphoramidite to prevent this side reaction. TIPS-5-Ethynyl-dU-CE Phosphoramidite, containing a protected alkyne, offers broader compatibility with oligonucleotide synthesis and deprotection. Protecting the 5-ethynyl group with a triisopropylsilyl (TIPS) protecting group prevents acid or base catalyzed hydration during oligonucleotide synthesis and workup. A quick treatment with TBAF removes the TIPS protecting group.

Formula: C₄₁H₄₇N₄O₈P

M.W.: 754.81

F.W.: 314.19

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: Use ammonium hydroxide and deprotect at room temperature as required by nucleobases. Compatible with UltraMild deprotection. Not compatible with methylamine deprotection.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 1-2 days

Please Note: All products of baseclick are patent protected and available in collaboration with baseclick. Baseclick GmbH has filed the following patent applications: 1. WO2006/117161, New labelling strategies for the sensitive detection of analytes. 2. WO2008/952775, Click Chemistry for the production of reporter molecules. Baseclick GmbH holds a worldwide license for the research market of the "Click Chemistry" patent from "The Scripps Research Institute": 3. WO03/101972, Copper-catalysed ligation of azides and acetylenes.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

品牌介绍

CambioCambio成立于1985年，是一家私人控股的英国公司，在为大学，大型制药，生物技术，政府机构，农业组织和环境设施的科研实验室提供优质分子生物学试剂和消耗品方面享有盛誉。Established in 1985, Cambio is a privately held UK company with an excellent reputation for the supply of quality Molecular Biology reagents and consumables to scientific research laboratories within universities, large pharma, biotechs, government agencies, agricultural organisations and environmental facilities.产品列表：No.品牌货号名称规格1cambioCA-1188A2-01 RRUCL2Anti Human Collagenase, monoclonal mouse0.1mg2cambioCA-1188-BS1Anti Human TIMP-1, polyclonal Rabbit0.1mg3cambioCA-1012Anti Streptokinase, polyclonal sheep1ml4cambioCA-605Anti Human Urokinase, polyclonal goat1ml5cambioCA-1046A c-terminusAnti Human Interleukin 5, polyclonal rabbit1ml6cambioCA-1075A c-terminusAnti Human Dopamine D2-3 Receptor, polyclonal rabbit1ml7cambioCA-1119Anti Human Cystic Fibrosis Protein, polyclonal rabbit1ml8cambioCA-1511BAnti MMP9 (Gelatinase B), polyclonal rabbit1 ml9cambioCA-972-FHuman Placental DNA (intact genomic) 1mg 10cambioCA-972-MHuman Placental DNA (intact genomic)1mg 11cambioCA-972-06Human Genomic DNA (sonicated)100mg12cambioPM-1025-01Alkaline Phosphatase1,000U13cambioPM-1025-02Alkaline Phosphatase5000U14cambioCA-972-FHuman Placental DNA (intact genomic)1mg15cambioCA-972-MHuman Placental DNA (intact genomic)1mg

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目录适体 Laryngopharyngeal Sampling Swab Resins sbeadex™ plant IMAC Agaroses Click Chemistry STEM Kits Broad Range Transfection Reagents sbeadex™ forensic

Apollo Accessories Gene Assays and Gene Panels sbeadex™ plasmid qRT-PCR Magnetic Beads Rest DNA Testing Reagents PCR Products His-tag Agaroses Purification Nasopharyngeal swabs qPCR主混合物 Apollo 7 mL Ultrafiltration Water Pathogen Testing Kits PCR Equipment 保护作用 TATAA Universal Spikes Amino Acids sbeadex™ PCR clean-up kit sbeadex™ livestock General transfection reagents Buccal Swabs Reagents & Linkers sbeadex™ pathogen nucleic acid purification Extra-long PCR 锑铅™ 组织 Glutathione Agarose Cell Lines Transposomics ™ Apollo 20 mL Ultrafiltration RNA合成 qPCR Cancer panels - Grand Perfomance Assays sbeadex™ blood 蛋白质提取试剂盒 Primary Cells STEM equipment

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