Description
DBCO-sulfo-NHS Ester
Catalog Number: 50-1941-xx
| 6-oxo-6-(dibenzo[b,f]azacyclooct-4-yn-1-yl)-caproic acid sulfo- N-hydroxysuccinimide ester, sodium salt | ||
| Formula: C25H21N2NaO8S | M.W.: 532.50 | F.W.: 316.37 |
COPPER-FREE CLICK CHEMISTRY
At Glen Research, our goal was to offer a copper-free click phosphoramidite reagent with the following properties:
Simple to useStable in solution on the synthesizerStable to ammonium hydroxide and AMAExcellent click performance in 17 hours or less at room temperature
From the variety of cyclooctyne-based copper-free click reagents so far described, we have chosen to offer compounds based on a dibenzo-cyclooctyne (DBCO) structure. We are offering 5’-DBCO-TEG Phosphoramidite for preparing oligos with a 5’-DBCO modification and DBCO-dT-CE Phosphoramidite for inserting a DBCO group at any position within the oligonucleotide. DBCO-sulfo-NHS Ester is also offered for post-synthesis conjugation reactions. DBCO-modified oligos may be conjugated with azides in organic solvents, such as DMSO, or aqeous buffers. Depending on the azide used, the reaction will go to completion in 4-17 hours at room temperature. Simple desalting on a Glen Gel-Pak™ leads to a product with virtually quantitative conjugation efficiency.
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