Description
Minerva Biolabs DNA Extraction Kits are used for the extraction of DNA from ordinary samples, like cell culture supernatants containing up to 106 cells, with high efficiency. Anyhow, other samples matrices may contain protein with a concentration above 10 mg/ml, like cell pellets, serum, or fermentation products. These samples may require an additional Proteinase K treatment to increase the overall DNA yield for highest sensitivity.
In combination with Minerva Biolabs´ DNA Extraction kits 10 µl of the Proteinase K solution is used for each sample after mixing the sample with Conditioner (MB DNA Extraction Kit) or Buffer A (Microsart AMP Extraction).
All lots of this product are routinely checked by the extraction of 10 CFU/ml of mycoplasma from a defined complex sample matrix in comparison to an untreated sample. The extracts were used as template for mycoplasma PCR with the Intego® Mycoplasma Kit for qPCR using a protocol for 10 µl sample volume.
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