The MiQuant® Residual DNA dPCR E. coli is designed for the quantitative detection of residual DNA from E. coli cells in chemical and biological matrices.

The MiQuant® Residual DNA dPCR E. coli is designed for use with the QIAGEN® QIAcuity® digital PCR (dPCR) system as a novel rapid, robust and sensitive method for the detection of residual host cell DNA.

Type of PCR

Digital PCR (dPCR)

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Recommended Use

To test product quality, the amount of residual DNA in a drug"s final dosage form must follow guidelines established by the World Health Organization (WHO), the U.S. Food and Drug Administration (FDA), the European Pharmacopoeia, and other regulatory agencies.

Kit Components

    dPCR E. coli Mix in aliquots of 24 reactions each

    Rehydration Buffer

    Positive Control DNA

    Internal Control DNA

    dPCR Grade Water

Package Size

Cat. No. 58-0101:

24 reactions

Cat. No. 58-0102:

4 x 24 reactions

Result Evaluation

Absolute Quantification

Required Consumables

    PCR reaction tubes

    PCR Clean™ (15-2025, 15-2001)

    PCR reaction tubes or plates

    MiQuant® dPCR Validation Standard E. coli (Cat. No. 58-0201)

Required Lab Device

    QIAcuity® digital PCR cycle

    QIAcuity® 24Well 26K Nanoplates

    Microcentrifuge for PCR reaction tubes

    Pipetting equipment

Shelf Life and Storage

The expiry date of the unopened package is marked on the package label. The kit components must be stored at2 to8°C until use. The rehydrated components must be store at ≤ -18°C for a maximum of one month.

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