Description

sbeadex™ blood kit

Next generation beads for DNA purification from blood

Blood samples can often vary considerably in quality, and this can lead to wide variations in the consistency and yield of extracted DNA. Molecular testing protocols, including genotyping and next-generation sequencing applications require reliable and high quality sample preparations.

DNA purification from blood protocol

sbeadex™ blood is a magnetic bead based DNA purification technology for blood samples of up to 200 µL volume. These proprietary superparamagnetic beads are double coated and include 2-binding steps and follow the standard workflow.

Click for larger Image

sbeadex™ blood kits have been developed as a high-throughput and cost-effective DNA isolation method. It supports automated DNA extractions using magnetic particle handlers (e.g. KingFisher™) as well as liquid handlers.

Standard blood sample (anticoagulants) and preparations are:

EDTA blood
Heparin blood
Citrate blood
Buffy coats

Applications for sbeadex™ blood kits

NGS: enzymatic fragmentation
Library/Cloning: suitable for digestion and ligation
qPCR/PCR: compatible with fluorescent BHQ probes and KASP chemistry
Sanger Sequencing

Advantages of a double-coated sbeadex blood purification kit

Efficient - purification of high molecular weight DNA from whole blood and buffy coat preparations
High quality – consistent OD_260/280 ≥ 1.8 ratio and an average OD_260/230 1.9 ratio
Easy to automate – magnetic microparticle handling offers flexibility
Absence of PCR inhibitors – no organic solvents or chaotropic salts in final wash buffers.
Convenient – all buffers supplied are ready to use
Optimised protocols - Less than 1 hour to DNA

Blood DNA purification kit specifications

Colour	Dark brown
Magnetic bead structure	Proprietary double-coated technology (competitor: single-coated)
Size and shape	Irregular Diameter: 80% 5 – 10 µm; all < 53 µm
Maximum capacity	2 µg DNA / mg sbeadex particle
Binding material and technology	2-step binding mechanism: first silica and second DNA adaptor
DNA Quality	Consistently high dsDNA content - Average 260/280: 1.8 and 260/230: 1.9
Purification format	Prepare in 1.5 mL tubes/96-well blocks
Automation	KingFisher (lab validated); applicable with alternative open liquid handlers
Sample Prep Time	Less than 1 hour from sample to purified DNA
Sample material	Whole blood; buffy coat; livestock; forensics; plasmids; other human samples
Compatibility	Anticoagulants: EDTA, Citrate, Heparin
Yield	From 4 – 12 µg Average yield 6.7 µg
Scale	100 µL to 200 µL tested with no effect on yield with an elution range from 50 – 200 µL
Purify from a range of blood storage conditions	Within 8 hours of blood collection and up to 3 years (-20 ° C) in storage
Applications	Suitable for digestion, ligation, cloning, PCR, qPCR NGS: Enzymatic fragmentation library pre-prep and size selection beads Compatible with KASP^®/qPCR/BHQ^® Probe MMx and BHQ Probe

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

品牌介绍

CambioCambio成立于1985年，是一家私人控股的英国公司，在为大学，大型制药，生物技术，政府机构，农业组织和环境设施的科研实验室提供优质分子生物学试剂和消耗品方面享有盛誉。Established in 1985, Cambio is a privately held UK company with an excellent reputation for the supply of quality Molecular Biology reagents and consumables to scientific research laboratories within universities, large pharma, biotechs, government agencies, agricultural organisations and environmental facilities.产品列表：No.品牌货号名称规格1cambioCA-1188A2-01 RRUCL2Anti Human Collagenase, monoclonal mouse0.1mg2cambioCA-1188-BS1Anti Human TIMP-1, polyclonal Rabbit0.1mg3cambioCA-1012Anti Streptokinase, polyclonal sheep1ml4cambioCA-605Anti Human Urokinase, polyclonal goat1ml5cambioCA-1046A c-terminusAnti Human Interleukin 5, polyclonal rabbit1ml6cambioCA-1075A c-terminusAnti Human Dopamine D2-3 Receptor, polyclonal rabbit1ml7cambioCA-1119Anti Human Cystic Fibrosis Protein, polyclonal rabbit1ml8cambioCA-1511BAnti MMP9 (Gelatinase B), polyclonal rabbit1 ml9cambioCA-972-FHuman Placental DNA (intact genomic) 1mg 10cambioCA-972-MHuman Placental DNA (intact genomic)1mg 11cambioCA-972-06Human Genomic DNA (sonicated)100mg12cambioPM-1025-01Alkaline Phosphatase1,000U13cambioPM-1025-02Alkaline Phosphatase5000U14cambioCA-972-FHuman Placental DNA (intact genomic)1mg15cambioCA-972-MHuman Placental DNA (intact genomic)1mg

公司介绍

品牌分类

目录适体 Laryngopharyngeal Sampling Swab Resins sbeadex™ plant IMAC Agaroses Click Chemistry STEM Kits Broad Range Transfection Reagents sbeadex™ forensic

Apollo Accessories Gene Assays and Gene Panels sbeadex™ plasmid qRT-PCR Magnetic Beads Rest DNA Testing Reagents PCR Products His-tag Agaroses Purification Nasopharyngeal swabs qPCR主混合物 Apollo 7 mL Ultrafiltration Water Pathogen Testing Kits PCR Equipment 保护作用 TATAA Universal Spikes Amino Acids sbeadex™ PCR clean-up kit sbeadex™ livestock General transfection reagents Buccal Swabs Reagents & Linkers sbeadex™ pathogen nucleic acid purification Extra-long PCR 锑铅™ 组织 Glutathione Agarose Cell Lines Transposomics ™ Apollo 20 mL Ultrafiltration RNA合成 qPCR Cancer panels - Grand Perfomance Assays sbeadex™ blood 蛋白质提取试剂盒 Primary Cells STEM equipment

显示分类

联络我们

服务热线：4000-520-616

（限工作日9:00-18:00）

QQ ：1570468124

手机：18915418616

官网：http://www.cambio.co.uk