Description
Molly the Labradoodle has surprised you with a litter of puppies! Students will use gel electrophoresis to examine the link between genotype and phenotype in Molly’s puppies. By tracking the inheritance of a single trait, students can determine the puppies’ father. This lab allows you to cover Mendelian genetics and the relationship between genotype and phenotype using an engaging and adorable case study. An extension activity allows students to practice tracking the inheritance of multiple genes that affect dog coats.
- Techniques: Micropipetting, gel electrophoresis
- Topics: Mendelian inheritance, genotype to phenotype, biotechnology
- Time required: Can be completed in one 45-minute class period
- Level: General high school through advanced high school
What is included
This lab kit contains reagents for eight lab groups:
- 8 puppy DNA samples (Astro, Buster, Chewy, Daisy, Elsa, Flora, Ginger, Hugo)
- 1 Fast DNA Ladder 1
Note: Reagents are compatible with blueGel™ or other standard DNA electrophoresis systems
Supplied by user:
The Learning Lab Companion Kit (catalog no. MP-KT-1510-01) contains all the consumables (except water) needed for 8 lab groups. These reagents and consumables can also be sourced independently.
- Reagents and consumables supplied by user:
- Agarose (electrophoresis grade)
- DNA stain (e.g., GelGreen™)
- Gel electrophoresis buffer (e.g., 1X TBE)
- Distilled or deionized H2O (to dilute buffer concentrate – not included in Lab Companion Kit)
- Microtubes
- Required equipment:
- Micropipettes and tips
- Gel electrophoresis and fluorescence visualization system
Storage notes:
- Reagents require freezer storage
- Reagents are stable for 12 months when stored in the freezer
Downloads
- miniPCR Dog Genetics Lab Teacher’s Guide
- miniPCR Dog Genetics Lab Student’s Guide
- miniPCR Dog Genetics Lab Classroom Slides
- Meet a dog geneticist: Interview with Dr. Elaine Ostrander
- Three ways to prepare agarose gels
Safety Data Sheet – Dog Genetics Lab
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200
