Electrophoresis Forensics Lab- Wrongfully Convicted?

J.M. was convicted of attempted homicide and is currently serving a life sentence. J.M. has always maintained his innocence, and after years of appeals, the court has approved his request for DNA analysis of the evidence from his case. In this lab students will use gel electrophoresis to examine newly collected DNA evidence from J.M.’s case. Will the DNA evidence prove that J.M. was wrongly convicted, or will it only implicate him further? This lab demonstrates the power that DNA analysis brings to forensic investigations.  An optional extension allows students to explore population genetics and the statistical analysis forensic scientists use to evaluate the strength of DNA evidence.

• Techniques: Micropipetting, gel electrophoresis
• Topics: Forensics, genetics, biotechnology
• Time required: Can be completed in one 45-minute class periods 
• Level: General high school through advanced high school

he miniPCR Electrophoresis Forensics Lab kit contains reagents for 8 lab groups of up to 4 students each (32 students):

• Victim DNA sample
• J.M. DNA sample
• DNA Evidence 1
• DNA Evidence 2
• DNA molecular weight marker (Fast DNA Ladder 1)

Note: Reagents are compatible with blueGel™ or other standard DNA electrophoresis systems

Supplied by user: 

The Learning Lab Companion Kit (catalog no. KT-1510-01) contains all the consumables (except water) needed for 8 lab groups. These reagents and consumables can also be sourced independently.

• Reagents and consumables supplied by user:

• • Agarose (electrophoresis grade)
  • DNA stain (e.g., GelGreen™)
  • Gel electrophoresis buffer (e.g., 1X TBE)
  • Distilled or deionized H2O (to dilute buffer concentrate – not included in Lab Companion Kit)
  • Microtubes 

• Required equipment:

• • Micropipettes and tips
  • Gel electrophoresis and fluorescence visualization system

Storage notes:

• Reagents require freezer storage 
• Reagents are stable for 12 months when stored in the freezer

Downloads

• miniPCR Electrophoresis Forensics Lab Teacher’s Guide
• miniPCR Electrophoresis Forensics Lab Student’s Guide
• Electrophoresis Forensics Lab Classroom Slides
• DNAdots on DNA fingerprinting
• Three ways to prepare agarose gels

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