Description
Highlights:
- Immediate live observation possible
- Ideal optical properties for transmitted light and fluorescence microscopy
- Optimum cell vitality
- Full serum compatibility
- Efficient, rapid protocol
- Cells can be fixed and dyed
- Compatible with oil immersion
μ-Proteofection Kit VI AB is specifically designed for the introduction of antibodies into cells under conditions necessary for live cell imaging. As with μ-Proteofection Kit VI, the kit combines the outstanding optical properties of μ-Slides VI from ibidi GmbH and the high-efficiency reagent for antibody proteofection PROTEOfectene® AB from Biontex. The kit provides the ideal conditions for high-resolution microscopic observation of living cells proteofected with antibodies.
This procedure enables mono- and polyclonal antibodies of all isotypes to be introduced successfully and without loss of binding capability, while cell development can be observed immediately in excellent optical quality. The use of fluorescencelabeled antibodies enables a variety of cell constituents to be marked and high-resolution microscopy to be carried out directly.
| Application | High-resolution microscopy for living mammalian cells proteofected with antibodies (live cell imaging) |
|---|---|
| Content | PROTEOfectene® AB, IgG-FITC labeled as positive control, µ-Slides VI (6 channels each) |
| Assays | 90 per kit |
| Shipping | At room temperature |
| Storage | 4°C PROTEOfectene® AB; ≤ –15°C IgG |
| Shelf life | 1/2 year (after date of delivery) |
| Product Group | Microfection |
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