Cell Line Information:

Designations: 293 [HEK-293]
Depositors: FL Graham
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: embryonic kidney
Applications: efficacy testing, transfection host, viruscide testing
Receptors: vitronectin
Tumorigenic: YES
Age: fetus
Comments: The cells contains Adenovirus 5 DNA from left ends of the viral genome. The line is excellent for titrating human adenoviruses. The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit. The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosome 19 (19q13.2)

The Transfection Reagent:

293 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on 293 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 293 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of 293 Cell Avalanche™ Transfection Reagent for transfection on 293 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into 293 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on 293 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for 293 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this 293 Cell Avalanche Transfection Reagent.   FIG. 2. 293 cells were transfected with GFP vector (pEGFP-N3) by using 293 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

293 Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200