Cell line Information:

Designations: 3T3-L1
Depositors: Massachusetts Institute of Technology
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: embryo
Cell Type: fibroblast
Cellular Products: triglycerides
Applications: transfection host
Receptors: insulin, expressed
Age: embryo
L1 is a continuous substrain of 3T3 (Swiss albino) developed through clonal isolation. The cells undergo a pre-adipose to adipose like conversion as they progress from a rapidly dividing to a confluent and contact inhibited state. A high serum content in the medium enhances fat accumulation [PubMed ID: 4426090].

The Transfection Reagent:

3T3-L1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting 3T3-L1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for 3T3-L1 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into 3T3-L1 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Simplest and straightforward protocols
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

 Data:FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH/3T3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH/3T3 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in 3T3-L1 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as 3T3-L1 Cell Avalanche Transfection Reagent.FIG. 2. 3T3-L1 cells were transfected with GFP vector (pEGFP-N3) by using 3T3-L1 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells:3T3-L1 Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.

NIH/3T3 Cell
BALB/3T3 clone A31
3T3-Swiss albino Cell
MDCK Cell
Vero Cell
293 Cell
MRC-5 Cell
IMR-90 Cell
COS-7 Cell
BHK-21 Cell
3197-3 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200