Cell Line Information:
Cell Line Name: ACHN (ATCC® CRL-1611™)
Organism: Homo sapiens, human  
Tissue: kidney, pleural effusion
Product Format: frozen  
Morphology: epithelial  
Culture Properties: adherent  
Disease: renal cell adenocarcinoma  
Age: 22 years  
Gender: male  
Ethnicity: Caucasian  
Tumorigenic: Yes
Applications: ACHN may be of use for antiproliferative studies using human interferons or interferon inducers.
Comments: Growth is inhibited by human interferon [Hogan, T. F., ]. ACHN is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:
ACHN Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on ACHN cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for ACHN cells. No other transfection reagents can match the efficiency, convenience, and gentleness of ACHN Cell Avalanche® Transfection Reagent for transfection on ACHN cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
 

Features:

• Unique formulation-maximize transfection performance in ACHN cells
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols to Increase experimental throughput
• Active in serum-containing medium
• Suitable for Reverse Transfection  
• Compatible with transfection in any plate format
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on ACHN cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for ACHN cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this ACHN Cell Avalanche® Transfection Reagent.

For Other Cells:

ACHN Cell Avalanche® Transfection Reagent (human kidney adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies. A498 Cell   RXF393 Cell   SN12C Cell   TK10 Cell   UO31 Cell   Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200