Cell Line Information:

Designations: AGS
Depositors: SC Barranco
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: stomach
Disease: gastric adenocarcinoma
Isolation date: 1979
Applications: transfection host
Tumorigenic: Yes
Age: 54 years
Gender: female
Ethnicity: Caucasian
Comments: The AGS cell line was derived from fragments of a tumor resected from a patient who had received no prior therapy. The cells have a plating efficiency of 34% in the medium below.

The Transfection Reagent:

AGS Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on AGS Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for AGS Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of AGS Cell Avalanche™ Transfection Reagent for transfection on AGS Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in AGS Cells.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on AGS cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for AGS cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this AGS Cell Avalanche Transfection Reagent.

For Other Cells:

AGS Cell Avalanche™ Transfection Reagent (human gastric adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies. NCI-N87 Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell PC-3 Cell AR42J Cell BxPC-3 Cell AsPC-1 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200