Cell line Informatrion:

Designations: BALB/3T3 clone A31
Depositors: S Aaronson
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: embryo
Strain: BALB/c
Cell Type: fibroblast
Isolation date: 1968
Applications: transfection host
Virus Susceptibility: Human poliovirus 1, Herpes simplex virus, Vesicular stomatitis virus
Tumorigenic: No
Age: embryo; 14 to 17 day gestation
The BALB/3T3 clone A31 is one of several cell lines developed by S.A. Aaronson and G.T. Todaro in 1968 from disaggregated 14- to 17-day-old BALB/c mouse embryos. The cells are extremely sensitive to contact inhibition of cell division, grow at a high dilution, exhibit a low saturation density and are highly susceptible to transformation in tissue culture by the oncogenic DNA virus SV40 and murine sarcoma virus.

The Transfection Reagent:

BALB/3T3 clone A31 Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting BALB/3T3 clone A31 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for BALB/3T3 clone A31 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into BALB/3T3 clone A31 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with serum
• Suitable for Reverse Transfection 
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH/3T3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH/3T3 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in BALB/3T3 clone A31 by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as BALB/3T3 clone A31 Avalanche Transfection Reagent.   FIG. 2. BALB/3T3 clone A31 cells were transfected with GFP vector (pEGFP-N3) by using BALB/3T3 clone A31 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

BALB/3T3 clone A31 Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. NIH/3T3 Cell 3T3-L1 Cell 3T3-Swiss albino Cell MDCK Cell Vero Cell 293 Cell MRC-5 Cell IMR-90 Cell COS-7 Cell BHK-21 Cell 3197-3 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200