Cell Line Information:

Designations: bEnd.3
Depositors: EC Butcher
Growth Properties: adherent
Organism: Mus musculus
Morphology: endothelial
Source: Organ: brain
Strain: BALB/c
Tissue: cerebral cortex
Disease: endothelioma
Cell Type: endothelialpolyoma middle T antigen transformed
Age: 6 weeks
Comments: The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen. The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL). The expression of Peyer"s Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd.3 cells by cytokines and lipopolysaccharide (LPS). Intracellular adhesion molecule 1 (ICAM-1) is constitutively expressed on the cells, and expression is increased by treatment with LPS, IL-1 and TNF alpha. Vascular cell adhesion molecule 1 (VCAM-1) is constitutively expressed on the cells at early passages but not at passages over 30.

The Transfection Reagent:

bEnd.3 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on bEnd.3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for bEnd.3 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of bEnd.3 Cell Avalanche™ Transfection Reagent for transfection on bEnd.3 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in bEnd.3 Cells.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on bEnd.3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for bEnd.3 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this bEnd.3 Cell Avalanche Transfection Reagent.

For Other Cells:

bEnd.3 Cell Avalanche™ Transfection Reagent (mouse brain endothelioma cell) can also be used on the following cells with high transfection efficiencies. 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200