Cell Line Information:

Designations: BHK-21Cell
Type: fibroblast
Isolation date: March, 1961
Applications: transfection host
Virus Susceptibility:  Human adenovirus, Human poliovirus, Reovirus, Vesicular stomatitis virus
Age:  1 day old newborn
HeLa Markers:  N
The parent line of BHK-21(C-13) was derived from the kidneys of five unsexed, 1-day-old hamsters in March, 1961, by I.A. Macpherson and M.G.P. Stoker. Following 84 days of continuous cultivation, interrupted only by an 8-day preservation by freezing, clone 13 was initiated by single-cell isolation. This line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs. The World Organization for Animal Health (OIE) uses the cells for routine diagnosis of rabies.

The Transfection Reagent:

BHK-21 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on BHK-21 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for BHK-21 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of BHK-21 Cell Avalanche™ Transfection Reagent for transfection on BHK-21 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in BHK-21 Cells.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Suitable for Reverse Transfection 
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on BHK-21 Cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for BHK-21 Cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this BHK-21 Cell Avalanche Transfection Reagent.   FIG. 2. BHK-21 cells were transfected with GFP vector (pEGFP-N3) by using BHK-21 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

BHK-21 Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. 786-O Cell Caki-1 Cell MDCK Cell BJ Cell Detroit 551 Cell MG-63 Cell WI-38 Cell MRC-5 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200