Cell Line Information:
Designations: CCRF-CEM
Organism: Homo sapiens (human)
Tissue: leukemia, acute lymphoblastic; peripheral blood; T lymphoblast
Age: 4 years
Gender: female
Ethnicity: caucasian
Morphology: lymphoblast
Growth properties: suspension
Tumorigenic: yes, in syrian hamsters
Reverse Transcriptase: negative
AntigenExp: CD3 B (37%), CD4 (50%), CD5 (95%), CD7 (77%)
Depositor: G.E. Foley
CCRF-CEM is a T lymphoblastoid cell line derived by G.E. Foley, et al. Cells were obtained in November, 1964 from peripheral blood buffy coat of a 4-year-old Caucasian female with acute lymphoblastic leukemia.

CCRF-CEM is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:

Transfection of suspension cells, such as CCRF-CEM Cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments.

CCRF-CEM Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting CCRF-CEM Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for CCRF-CEM Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into CCRF-CEM Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on CCRF-CEM cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for CCRF-CEM cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this CCRF-CEM Cell Avalanche Transfection Reagent.

For Other Cells:

CCRF-CEM Cell Avalanche™ Transfection Reagent (human leukemia cell) can also be used on the following cells with high transfection efficiencies. 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell MRC-5 Cell IMR-90 Cell COS-7 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200