Cell Line Information:

Designations: CHO
Organism: Cricetulus griseus (hamster, Chinese)
Tissue: ovary
Gender: female
Morphology: epithelial-like
Growth properties: adherent
VirusSuscept: vesicular stomatitis (Indiana); Getah virus
VirusResist: poliovirus 2; modoc virus; Button Willow virus
Reverse Transcriptase: negative
Depositors: T.T. Puck
Chinese hamster ovary (CHO) cells are a cell line derived from the ovary of the Chinese hamster. The CHO line was established in 1957. The Chinese hamster has a low chromosome number (2n=22) making it an excellent model for radiation cytogenetics and tissue culture. CHO are the most widely used mammalian cells for transfection, expression, and large-scale recombinant protein production. The cell line is highly sensitive to toxic material. The CHO clonal cytotoxicity test system has been validated through analysis of organic compounds, environmental samples, and metal salts.

The Transfection Reagent:

CHO Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on CHO Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for CHO Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of CHO Cell Avalanche™ Transfection Reagent for transfection on CHO Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in CHO Cells.
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Suitable for Reverse Transfection 
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on CHO-K1 Cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for CHO-K1 Cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this CHO Cell Avalanche Transfection Reagent.   FIG. 2. CHO-K1 cells were transfected with GFP vector (pEGFP-N3) by using CHO Cells Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

CHO Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. SK-OV-3 Cell Vero Cell 293 Cell 293T/17 Cell Saos-2 Cell SK-N-SH Cell B16-F10 Cell 4T1 Cell A549 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200