Description
Cell Line Information:
Designations: COLO 205
Depositors: GE Moore
Growth Properties: mixed, adherent and suspension
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: colon
Tumor Stage: Dukes" type D
Disease: colorectal adenocarcinoma
Derived from metastatic site: ascites
Cellular Products: carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10)
Applications: transfection host
Tumorigenic: Yes
Age: 70 years
Gender: male
Ethnicity: Caucasian
Comments: This line was isolated in 1975 by T.U. Semple, et al. from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The cells are positive for keratin by mmunoperoxidase staining. COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.
COLO 205 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.
The Transfection Reagent:
Transfection of suspension cells, or suspension/adherent mixed cells, such as COLO 205 Cells, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.
COLO 205 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting COLO 205 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for COLO 205 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into COLO 205 Cells
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- Deliver single or multiple plasmids
- Simplest and straightforward protocols
- Compatible with serum
- Suitable for Reverse Transfection
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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COLO 205 Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies. 786-O Cell |
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