Cell Line Information:

Designations: Daudi
Depositors: G Klein
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: peripheral blood
Disease: Burkitt"s lymphoma
Cell Type: B lymphoblast;
Isolation: Isolation date: May, 1967
Applications: transfection host
Receptors: complement,Fc
Tumorigenic: Yes
Isoenzymes: G6PD, B
Age: 16 years
Gender: male
Ethnicity: Black
The Daudi is a well characterized B lymphoblast cell line which has been employed extensively in studies of mechanisms of leukemogenesis.

The Transfection Reagent:

Transfection of suspension cells, such as Daudi Cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments.

Daudi Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Daudi Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Daudi Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of Daudi Cell Avalanche™ Transfection Reagent for transfection on Daudi Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into Daudi Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Simplest and straightforward protocols
• Compatible with serum
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Daudi cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Daudi cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Daudi Cell Avalanche Transfection Reagent.

For Other Cells:

Daudi Cell Avalanche™ Transfection Reagent (human lymphoma cell) can also be used on the following cells with high transfection efficiencies. Vero Cell 293 Cell VCaP Cell PC-3 Cell AR42J Cell Capan-1 Cell PANC-1 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200