Cell Line Information:
Designations: Detroit 551
Organism: Homo sapiens (human)
Tissue: skin; fibroblast
Age: fetus
Gender: female
Ethnicity: Caucasian
VirusSuscept: herpes simplex; SV40; poliovirus 1; vesicular stomatitis (Indiana)
Morphology: fibroblast
Growth properties: adherent
Reverse Transcriptase: negative
Depositors: C. S. Stulberg
The cells have a finite lifespan of about 25 serial passages from the tissue of origin. They can be transformed by SV40. Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.
Karyotype: normal human female; diploid

The Transfection Reagent:

Detroit 551 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Detroit 551 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Detroit 551 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into Detroit 551 Cells
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on BJ cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for BJ cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in Detroit 551 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as Detroit 551 Cell Avalanche Transfection Reagent.   FIG. 2. Detroit 551 cells were transfected with GFP vector (pEGFP-N3) by using Detroit 551 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

Detroit 551 Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. Vero Cell 293 Cell 293T/17 Cell IMR-90 Cell COS-7 Cell BHK-21 Cell 3197-3 Cell NIH/3T3 Cell 3T3-L1 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200