Cell Line Information:

Designations: HCC1937
Depositors: AF Gazdar, AK Virmani
Growth Properties: adherent, The line grows as large epithelial cells with a tendency to float at high cell densities
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: mammary gland; breast
Tissue: duct
Tumor Stage: TNM stage IIB, grade 3
Disease: primary ductal carcinoma
Cellular Products: Epithelial glycoprotein 2 (EGP2), cytokeratin 19
Isolation date: October 13, 1995
progesterone receptor, negative
Oncogene: BRCA1 (mutated, insertion C at nucleotide 5382), her2/neu -, p53 -.
Age: 23 years adult
Gender: female
Ethnicity: Caucasian
Comments: This cell line was initiated from a primary ductal carcinoma on October 13, 1995, and took 11.5 months to establish. The cell line has an acquired homozygous deletion of the PTEN gene, and loss of heterozygosity at multiple loci known to be involved in the pathogenesis of breast cancer.

The Transfection Reagent:

HCC1937 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HCC1937 Cells. The proprietary formulation of lipids and polymers ensures the maximum possible transfection efficiency and cell viability. No other transfection reagents can match the efficiency, convenience, and gentleness of HCC1937 Cell Avalanche™ Transfection Reagent for transfection on HCC1937 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in HCC1937 Cells.
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on ZR-75-1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for ZR-75-1 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on HCC1937 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as HCC1937 Cell Avalanche Transfection Reagent.   FIG. 2. HCC1937 cells were transfected with GFP vector (pEGFP-N3) by using HCC1937 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

For Other Cells:

HCC1937 Cell Avalanche™ Transfection Reagent (human breast carcinoma cell) can also be used on the following cells with high transfection efficiencies. 4T1 Cell ZR-75-1 Cell BT-20 Cell MDA-MB-453 Cell MDA-MB-468 Cell SK-BR-3 Cell MDA-MB-231 Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200