Cell Line Information:

Designations: HeLa
Depositors: WF Scherer
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: cervix
Disease: adenocarcinoma
Cell Type: epithelial
Applications: transfection host, screening for Escherichia coli strains with invasive potential
Virus Susceptibility: Human adenovirus 3, Encephalomyocarditis virus
Isoenzymes: G6PD, A
Age: 31 years adult
Gender: female
Ethnicity: Black
HeLa Markers: Y
Comments: The cells are positive for keratin by immunoperoxidase staining. HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. P53 expression was reported to be low, and normal levels of pRB (retinoblastoma suppressor) were found.

The Transfection Reagent:

Hela Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Hela Cell. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Hela Cell. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into Hela Cell
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Hela cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Hela cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Hela Cell Avalanche Transfection Reagent.   FIG. 2. Hela cells were transfected with GFP vector (pEGFP-N3) by using Hela cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

Hela Cell Avalanche™ Transfection Reagent (human cervix adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies. HEp-2 Cell 293 Cell 293T/17 Cell 786-O Cell DU 145 Cell 22Rv1 Cell VCaP Cell PC-3 Cell AR42J Cell Capan-1 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200