Cell Line Information:

Designations: Hep 3B
Morphology: epithelial
Growth Properties: adherent
Organ: liver
Applications: Transfection host
Disease: hepatocellular carcinoma
Cellular Products: alpha fetoprotein (alpha-fetoprotein); hepatitis B surface antigen (HBsAg); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); [3525] alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein); Gc globulin [3525]
Organism: Homo sapiens
Age: 8 years juvenile
Gender: male
Ethnicity: Black

The Transfection Reagent:

Hep 3B Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Hep 3B Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Hep 3B Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of Hep 3B Cell Avalanche™ Transfection Reagent for transfection on Hep 3B Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into Hep 3B Cells.
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Hep G2 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Hep G2 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on Hep 3B cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as Hep 3B Cell Avalanche Transfection Reagent.   FIG. 2. Hep 3B cells were transfected with GFP vector (pEGFP-N3) by using Hep 3B Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

Hep 3B Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell) can also be used on the following cells with high transfection efficiencies. Hepa 1-6 Cell Hep G2 Cell HepG2/C3A Cell PANC-1 Cell BxPC-3 Cell AsPC-1 Cell CHO-K1 Cell SK-OV-3 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200