Cell Line Information:

Designations: Hep G2 
Morphology: epithelial
Organ: liver
Disease: hepatocellular carcinoma
Cellular Products: alpha-fetoprotein (alpha fetoprotein); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin);
Age: 15 years adolescent
Gender: male
Ethnicity: Caucasian
The cells express 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities. The cells demonstrate decreased expression of apoA-I mRNA and increased expression of catalase mRNA in response to gramoxone (oxidative stress).

The Transfection Reagent:

Hep G2 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Hep G2 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Hep G2 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of Hep G2 Cell Avalanche™ Transfection Reagent for transfection on Hep G2 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in Hep G2 Cells.
• Extremely gentle to cells
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Hep G2 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Hep G2 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Hep G2 Cell Avalanche Transfection Reagent.   FIG. 2. Hep G2 cells were transfected with GFP vector (pEGFP-N3) by using Hep G2 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

Hep G2 Cell Avalanche™ Transfection Reagent (human hepatocellular carcinoma cell) can also be used on the following cells with high transfection efficiencies. Hepa 1-6 Cell Hep 3B Cell HepG2/C3A Cell PANC-1 Cell BxPC-3 Cell AsPC-1 Cell CHO-K1 Cell SK-OV-3 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200