Cell Line Information:

Designations: Hepa 1-6
Depositors: GJ Darlington
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Source: Organ: liver
Strain: C57L
Disease: hepatoma
Cellular Products: albumin, alpha fetoprotein (AFP, alpha-fetoprotein); albumin; alpha 1 antitrypsin (alpha-1-antitrypsin); amylase
Applications: transfection host
Comments: This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse.

The Transfection Reagent:

Hepa 1-6 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Hepa 1-6 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Hepa 1-6 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into Hepa 1-6 Cells
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Efficient for plasmid DNA/siRNA co-transfection
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

 Data:FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HEPA 1-6 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HEPA 1-6 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this HEPA 1-6 Cell Avalanche Transfection Reagent.FIG. 2. Hepa 1-6 cells were transfected with GFP vector (pEGFP-N3) by using Hepa 1-6 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells:Hepa 1-6 Cell Avalanche™ Transfection Reagent (mouse hepatoma cell) can also be used on the following cells with high transfection efficiencies.

Hep G2 Cell
Hep 3B Cell
HepG2/C3A Cell
PANC-1 Cell
BxPC-3 Cell
AsPC-1 Cell
CHO-K1 Cell
SK-OV-3 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200