Cell Line Information:

Designations: HT-1080
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: connective tissue
Disease: fibrosarcoma
Isolation date: July, 1972
Applications: transfection host
Virus Susceptibility:Human poliovirus 1, RD-114 Feline, Feline leukemia virus, Vesicular stomatitis virus
Tumorigenic: Yes
Oncogene: ras
Isoenzymes: G6PD, B
Age: 35 years
Gender: male
Ethnicity: Caucasian
The cells contain an activated N-ras oncogene.

The Transfection Reagent:

HT-1080 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HT-1080 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HT-1080 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HT-1080 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Compatible with transfection in any plate formats
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HT-1080 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HT-1080 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this HT-1080 Cell Avalanche Transfection Reagent.

For Other Cells:

HT-1080 Cell Avalanche™ Transfection Reagent (human fibrosarcoma cell) can also be used on the following cells with high transfection efficiencies. Caki-1 Cell MDCK Cell Vero Cell 293 Cell PC-3 Cell AR42J Cell Capan-1 Cell PANC-1 Cell BxPC-3 Cell AsPC-1 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200