Cell Line Information:

Designations: J774A.1
Depositors: P Ralph 
Growth Properties: adherent
Organism: Mus musculus
Morphology: macrophage
Source: Strain: BALB/cN
Tissue: ascites
Disease: reticulum cell sarcoma
Cell Type: monocyte/macrophage
Cellular Products: interleukin 1 beta lysozyme
Isolation: La Jolla California, United States
Isolation date: 1968
Applications: Biological response; transfection host
Age: adult
Gender: female
J774A.1 cells are active in antibody dependent phagocytosis. Their growth is inhibited by dextran sulfate, PPD and LPS. They synthesize large amounts of lysozyme and exhibits minor cytolysis but predominantly antibody-dependent phagocytosis. Interleukin 1 beta (Il1b) is synthesized continuously by this line.

The Transfection Reagent:

J774A.1 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on J774A.1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for J774A.1 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of J774A.1 Cell Avalanche™ Transfection Reagent for transfection on J774A.1 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in J774A.1 Cells.
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Animal-free
• Simplest and straightforward protocols
• Compatible with serum
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems 

 Data:FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on J774A.1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for J774A.1 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this J774A.1 Cell Avalanche Transfection Reagent.
For Other Cells:J774A.1 Cell Avalanche™ Transfection Reagent (mouse reticulum cell sarcoma) can also be used on the following cells with high transfection efficiencies.

RAW 264.7 Cell
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.      Research Use Only. Not for use in diagnostic or therapeutic procedures.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200