Cell Line Description:

Designations: Jurkat, Clone E6-1
Depositors: A Weiss
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: acute T cell leukemia
Cell Type: T lymphocyte;
Cellular Products: interleukin-2 (interleukin 2, IL-2)
Applications: transfection host
Receptors: T cell antigen receptor, expressed
Antigen Expression: CD3; Homo sapiens, expressed
Gender: male
This is a clone of the Jurkat-FHCRC cell line, a derivative of the Jurkat cell line. The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM.
Clone E6-1 cells produce large amounts of IL-2 after stimulation with phorbol esters and either lectins or monoclonal antibodies against the T3 antigen (both types of stimulants are needed to induce IL-2 production).

The Transfection Reagent:

Transfection of suspension cells, such as Jurkat-FHCRC Cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments.

Jurkat-FHCRC Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Jurkat-FHCRC Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Jurkat-FHCRC Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in Jurkat-FHCRC Cells.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Jurkat-FHCRC cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Jurkat-FHCRC cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Jurkat-FHCRC Cell Avalanche Transfection Reagent.

For Other Cells:

Jurkat-FHCRC Cell Avalanche™ Transfection Reagent (human leukemia cell) can also be used on the following cells with high transfection efficiencies. 293 Cell 293T/17 Cell DU 145 Cell 22Rv1 Cell VCaP Cell Capan-1 Cell PANC-1 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200