Cell Information:

Designations: K-562 
Depositors: HT Holden
Growth Properties: suspension
Organism: Homo sapiens
Morphology:lymphoblast
Organ: bone marrow
Disease: chronic myelogenous leukemia (CML)
Applications: transfection host
Tumorigenic: Yes
Antigen Expression: CD7 (25%)
Age: 53 years
Gender: female
The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises.
The cell population has been characterized as highly undifferentiated and of the granulocytic series. Studies conducted by Anderson, et al., on the surface membrane properties led to the conclusion that the K-562 was a human erythroleukemia line.
The K-562 cell line has attained widespread use as a highly sensitive in vitro target for the natural killer assay. K-562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the erythrocytic, granulocytic and monocytic series.

K-562 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:

Transfection of suspension cells, such as K-562 Cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments.

K-562 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on K-562 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for K-562 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of K-562 Cell Avalanche™ Transfection Reagent for transfection on K-562 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in K-562 Cells.
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Animal-free
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on K-562 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for K-562 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this K-562 Cell Avalanche Transfection Reagent.

For Other Cells:

K-562 Cell Avalanche™ Transfection Reagent (human leukemia cell) can also be used on the following cells with high transfection efficiencies. Caki-1 Cell 293 Cell 293T/17 Cell NIH/3T3 Cell 3T3-L1 Cell CT26.WT Cell NCTC Clone 929 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200