Cell Line Information:
Cell Line Name: KM12
Prior Treatment: No
Tissue Type: Colon
Histology: Adenocarcinoma-pd; epithelium
p53 Status: 0
ECAD Methylation: 17
MDR Function: -19
Institution: M.D. Anderson Hospital & Tumor Institute
Contributor: Fidler, IJ
Reference: Cancer Res. 48:1943-1948, 1988
KM12 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:
KM12 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on KM12 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for KM12 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of KM12 Cell Avalanche® Transfection Reagent for transfection on KM12 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
 

Features:

• Unique formulation-maximize transfection performance in KM12 cells
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols to Increase experimental throughput
• Active in serum-containing medium
• Suitable for Reverse Transfection  
• Compatible with transfection in any plate format
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on KM12 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for KM12 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this KM12 Cell Avalanche® Transfection Reagent.

For Other Cells:

KM12 Cell Avalanche® Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies. HCC2998 Cell   HCT-15 Cell T84 Cell DLD-1 Cell HT-29 Cell Caco-2 Cell LoVo Cell SW480 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200