Cell Line Information:
Designations: L6
Organism: Rattus norvegicus (rat)
Tissue: skeletal muscle; myoblast
Morphology: myoblast
Growth properties: adherent
Products: myosin
Depositors: D. Schubert
The L6 myogenic line was isolated originally by Yaffe from primary cultures of rat thigh muscle maintained for the first two passages in the presence of methyl cholanthrene.
L6 cells fuse in culture to form multinucleated myotubes and striated fibers. The myoblastic component of this line will be depleted rapidly if the cells are allowed to become confluent.

The Transfection Reagent:

L6 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on L6 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for L6 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of L6 Cell Avalanche™ Transfection Reagent for transfection on L6 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into L6 Cells
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on L6 Cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for L6 Cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this L6 Cell Avalanche Transfection Reagent.

For Other Cells:

L6 Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. H9c2(2-1) C2C12 Cell 786-O Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200