Cell Line Information:
Cell Line Name: Malme-3M (ATCC® HTB-64™)
Organism: Homo sapiens, human
Tissue Derived from metastatic site: lung
Cell Type: fibroblast
Morphology: mixed
Culture Properties: mixed adherent-suspension
Biosafety Level; 1
Disease: Malignant melanoma
Age: 43 years
Gender: male
Ethnicity: Caucasian
Applications: This melanoma cell line was isolated from the same patient as Malme-3 (ATCC HTB-102), a normal skin fibroblast. The two cell lines provide tumor and normal counterparts for comparative in vitro  melanoma studies. This is one of an extensive series of human tumor lines isolated and characterized by J. Fogh.
Markers: HLA A2, Aw30, B13, B40( /-), DRw7
Tumorigenic: Yes
Malme-3M  is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:
Transfection of suspension cells, or suspension/adherent mixed cells, such as Malme-3M cell, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Malme-3M Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Malme-3M cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Malme-3M cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in Malme-3M cells
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Free of animal-derived components
• Simplest and straightforward protocols to Increase experimental throughput
• Active in serum-containing medium
• Compatible with transfection in any plate format
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Great pricing
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Malme-3M cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Malme-3M cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Malme-3M Cell Avalanche® Transfection Reagent.

For Other Cells:

Malme-3M Cell Avalanche® Transfection Reagent (human melanoma cell) can also be used on the following cells with high transfection efficiencies. LOX-IMVI Cell   M14 Cell SK-MEL-2 Cell SK-MEL-5 Cell   UACC-257 Cell   UACC-62 Cell   Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200