Cell Line Information:

Designations: MDA-MB-453
Depositors: R Cailleau
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: mammary gland; breast
Disease: metastatic carcinoma
Derived from metastatic site: pericardial effusion
Isolation date: June 10, 1976
Applications: transfection host
Receptors: fibroblast growth factor (FGF), expressed
Tumorigenic: No
Age: 48 years
Gender: female
Ethnicity: Caucasian
Comments: MDA-MB-453 was derived in 1976 by R. Cailleau et al. from an effusion of a 48 year old female patient with metastatic carcinoma of the breast, involving the nodes, brain and both pleural and pericardial cavities. The cells overexpress FGF receptors.

The Transfection Reagent:

MDA-MB-453 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on MDA-MB-453 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for MDA-MB-453 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of MDA-MB-453 Cell Avalanche™ Transfection Reagent for transfection on MDA-MB-453 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in MDA-MB-453 Cells.
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Efficient for plasmid DNA/siRNA co-transfection
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on MDA-MB-453 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for MDA-MB-453 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this MDA-MB-453 Cell Avalanche Transfection Reagent.   FIG. 2. MDA-MB-453 cells were transfected with GFP vector (pEGFP-N3) by using MDA-MB-453 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

For Other Cells:

MDA-MB-453 Cell Avalanche™ Transfection Reagent (human breast carcinoma cell) can also be used on the following cells with high transfection efficiencies. 4T1 Cell ZR-75-1 Cell BT-20 Cell HCC1937 Cell MDA-MB-468 Cell SK-BR-3 Cell MDA-MB-231 Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200