Cell Line Information:

Designations: MIA PaCa-2
Organism: Homo sapiens (human)
Tissue: carcinoma; pancreas
Age: 65 years
Gender: male
Ethnicity: Caucasian
Morphology: epithelial -like cells with clusters of rounded cells in suspension
Growth properties: adherent with many cells in suspension
Products: human colony stimulating factor, subclass I (CSF-I); plasminogen activator
Depositos: A. Yunis
The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%. The line is sensitive to asparaginase.

The Transfection Reagent:

Transfection of suspension cells, or suspension/adherent mixed cells, such as MIA PaCa-2 Cells, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

MIA PaCa-2 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on MIA PaCa-2 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for MIA PaCa-2 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of MIA PaCa-2 Cell Avalanche™ Transfection Reagent for transfection on MIA PaCa-2 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in MIA PaCa-2 Cells.
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

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