Cell Line Information: 

Designations: MRC-5
Depositors: JP Jacobs
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Organ: lung
Disease: normal
Cell Type: fibroblast
Isolation date: September, 1966
Applications: transfection host; viruscide testing
Isoenzymes: G6PD, B
Age: 14 weeks gestation
Gender: male
Ethnicity: Caucasian
The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male fetus by J.P. Jacobs in September of 1966. The cells are capable of 42 to 46 population doublings before the onset of senescence.

The Transfection Reagent:

MRC-5 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on MRC-5 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for MRC-5 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of MRC-5 Cell Avalanche™ Transfection Reagent for transfection on MRC-5 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into MRC-5 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Developed and manufactured by EZ Biosystems

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