Cell Line Information:

Designations: NCI-H1299
Organism: Homo sapiens (human)
Tissue: lung carcinoma; non-small cell lung cancer; derived from metastatic site: lymph node
Age: 43 years
Gender: male
Ethnicity: Caucasian
Morphology: epithelial
Growth properties: adherent
Products: neuromedin B (NMB)
Depositors: A.F. Gazdar; J.D. Minna
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis. The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. The cells are able to synthesize the peptide NMB at 0.1 pmol/mg protein, but not the gastrin releasing peptide (GRP).

The Transfection Reagent:

NCI-H1299 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NCI-H1299 Cells. The proprietary formulation of lipids and polymers ensures the maximum posssible transfection efficiency and cell viability. No other transfection reagents can match the efficiency, convenience, and gentleness of NCI-H1299 Cell Avalanche™ Transfection Reagent for transfection on NCI-H1299 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in NCI-H1299 Cells.
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on A549 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for A549 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on NCI-H1299 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as NCI-H1299 Cell Avalanche Transfection Reagent.

For Other Cells:

NCI-H1299 Cell Avalanche™ Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies. A549 Cell NCI-H292 Cell NCI-H460 Cell NCI-H23 Cell NCI-H1975 Cell NCI-H441 Cell Calu-3 Cell NCI-H358 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200