Cell Line Informtion:

Designations: NCI-H23
Depositors: AF Gazdar, JD Minna
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Organ: lung
Disease: adenocarcinoma; non-small cell lung cancer
Oncogene: myc; src; abl; erb; ras; sis -
Age: 51 years
Gender: male
Ethnicity: Black 
NCI-23 was derived from a lung cancer obtained from a patient prior to therapy. The line exhibits a high degree of c-myc DNA amplification (20-fold) but no detectable amplification of c-myc RNA. The cells stain positive for keratins 5 8 and 18 and vimentin but are negative for neurofilament.

NIH-H23 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:

NCI-H23 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on NCI-H23 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NCI-H23 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of NCI-H23 Cell Avalanche™ Transfection Reagent for transfection on NCI-H23 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in NCI-H23 Cells.
• Extremely gentle to cells
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with serum
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on A549 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for A549 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on NCI-H23 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as NCI-H23 Cell Avalanche Transfection Reagent.

For Other Cells:

NCI-H23 Cell Avalanche™ Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies. A549 Cell NCI-H292 Cell NCI-H460 Cell NCI-H1299 Cell NCI-H1975 Cell NCI-H441 Cell Calu-3 Cell NCI-H358 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200