Cell Line Information:

Designations: NCI-H292
VirusSuscept: hepatitis B virus (HBV)
Tumorigenic: yes, in nude mice; tumors histologically resemble the original biopsy specimen and retain mucoepidermoid features
Products: keratin; vimentin
DoublingTime: 48 hrs
Depositors: A.F. Gazdar
Comments: This line was derived from a lymph node metastasis of a pulmonary mucoepidermoid carcinoma. The cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation. The line has been selected as a prototype for transfecting human subgenomic fragments into human cells for studying the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer. The cells are mucicarmine positive but are negative for neurofilament triplet protein.

The Transfection Reagent:

NCI-H292 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NCI-H292 Cells. The proprietary formulation of lipids and polymers ensures the maximum possible transfection efficiency and cell viability. No other transfection reagents can match the efficiency, convenience, and gentleness of NCI-H292 Cell Avalanche™ Transfection Reagent for transfection on NCI-H292 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into NCI-H292 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.

 Data:FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on A549 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for A549 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on NCI-H292 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as NCI-H292 Cell Avalanche Transfection Reagent.
For Other Cells:NCI-H292 Cell Avalanche™ Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies.

A549 Cell
NCI-H460 Cell
NCI-H1299 Cell
NCI-H23 Cell
NCI-H1975 Cell
NCI-H441 Cell
Calu-3 Cell
NCI-H358 Cell
293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200