Cell Line Information:
Cell Line Name: NCI-H322M, NCI.H322M
Patient"s Age: 52
Patient"s Sex: M
Ethnicity: Caucasian
Prior Treatment: No
Tissue Type: Lung
Histology: Small cell bronchoalveolar adenocarcinoma; epithelium
p53 Status: 0
ECAD Methylation: 25
MDR Function: -10 Institution: Developmental Therapeutics Program, National Cancer Institute, Frederick,MD 21701
NCI-H322M is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:
NCI-H322M Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NCI-H322M cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NCI-H322M cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in NCI-H322M cells
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols (no optimization required)
• Compatible with serum and antibiotics
• Suitable for Reverse Transfection  
• Compatible with transfection in any plate format
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NCI-H322M cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NCI-H322M cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NCI-H322M Cell Avalanche® Transfection Reagent.

For Other Cells:

NCI-H322M Cell Avalanche® Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies. EKVX Cell   HOP-92 Cell   NCI-H226 Cell   NCI-H460 Cell NCI-H23 Cell NCI-H1975 Cell NCI-H441 Cell NCI-H358 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200