Cell Line Information:

Designations: NCI-H358 [H-358, H358]
Depositors: AF Gazdar, JD Minna
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Tissue: bronchiole; alveolus
Disease: bronchioalveolar carcinoma; non-small cell lung cancer
Cellular Products: lung surfactant associated protein A (SP-A)
Isolation date: 1981
Tumorigenic: Yes
Gender: male
Comments: This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells. The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.

The Transfection Reagent:

NCI-H358 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NCI-H358 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NCI-H358 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in NCI-H358 Cells.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Simplest and straightforward protocols
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

 Data:FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on A549 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for A549 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on NCI-H358 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as NCI-H358 Cell Avalanche Transfection Reagent.
For Other Cells:NCI-H358 Cell Avalanche™ Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies.

A549 Cell
NCI-H292 Cell
NCI-H460 Cell
NCI-H1299 Cell
NCI-H23 Cell
NCI-H1975 Cell
NCI-H441 Cell
Calu-3 Cell
293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200