Cell Line Information:
Cell Line Name: NCI-H522
Organism: Homo sapiens, human
Tissue: lung
Morphology: epithelial
Culture Properties: adherent, single cells and loosely attached clusters
Biosafety Level: 1
Disease: stage 2, adenocarcinoma; non-small cell lung cancer
Age: 58 years
Gender: male
Ethnicity: Caucasian
Comments: The cells carry the K-ras 12 mutation, and there is a mutation in codon 191 of the p53 gene.The cells are not able to synthesize the peptide neuromedin B (NMB) or the gastrin releasing peptide (GRP).The cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein.
Institution: University of Texas Southwestern Medical Center
Contributor: This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lung cancer obtained from a patient prior to therapy. The patient was a smoker (60-pack-year).
NCI-H522 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:
NCI-H522 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on NCI-H522 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NCI-H522 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of NCI-H522 Cell Avalanche® Transfection Reagent for transfection on NCI-H522 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
 

Features:

• Specifically optimized to deliver nucleic acids into NCI-H522 cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Free of animal-derived components
• Simplest and straightforward protocols to Increase experimental throughput
• Compatible with serum
• Active in serum-containing medium
• Suitable for Reverse Transfection  
• Great pricing
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NCI-H522 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NCI-H522 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NCI-H522 Cell Avalanche® Transfection Reagent.

For Other Cells:

NCI-H522 Cell Avalanche® Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies. EKVX Cell   HOP-92 Cell   NCI-H226 Cell   NCI-H322M Cell A549 Cell NCI-H23 Cell NCI-H1975 Cell Calu-3 Cell NCI-H358 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200