Cell Line Information:

Designations: NCTC clone 929 [L cell, L-929, derivative of Strain L]
Depositors: WR Earle
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: Tissue: subcutaneous connective tissue; areolar and adipose
Strain: C3H/An
Isolation date: March, 1948
Applications: toxicity testing, transfection host
Virus Resistance: poliovirus 1, 2, 3; coxsackievirus B5; polyomavirus
Tumorigenic: Yes
Antigen Expression: H-2k
Age: 100 days
Gender: male
Comments: NCTC clone 929 (Connective tissue, mouse) Clone of strain L was derived in March, 1948. Strain L was one of the first cell strains to be established in continuous culture, and clone 929 was the first cloned strain developed. The parent L strain was derived from normal subcutaneous areolar and adipose tissue of a 100-day-old male C3H/An mouse. Clone 929 was established (by the capillary technique for single cell isolation) from the 95th subculture generation of the parent strain.

The Transfection Reagent:

NCTC Clone 929 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NCTC Clone 929 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NCTC Clone 929 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in NCTC Clone 929 Cells.
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Efficient for plasmid DNA/siRNA co-transfection
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Suitable for Reverse Transfection 
• Compatible with transfection in any plate formats
• the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NCTC CLONE 929 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NCTC CLONE 929 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NCTC CLONE 929 Cell Avalanche Transfection Reagent.   FIG. 2. NCTC Clone 929 cells were transfected with GFP vector (pEGFP-N3) by using NCTC Clone 929 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

NCTC Clone 929 Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. Vero Cell 293 Cell MRC-5 Cell IMR-90 Cell COS-7 Cell BHK-21 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200