Cell Line Information:
Designations: Neuro-2a 
Organism: Mus musculus (mouse)
Strain: A
Genotype: albino
Tissue: brain; neuroblast; neuroblastoma
Morphology: neuronal and amoeboid stem cells
Growth properties: adherent
Antigen Expression: H-2a
Cellular products: tubulin; acetylcholinesterase
Depositor: R.J. Klebe 
Neuro-2a was established by R.J. Klebe and F.H. Ruddle from a spontaneous tumor of a strain A albino mouse. Neuro-2a cells produce large quantities of microtubular protein which is believed to play a role in a contractile system which is responsible for axoplasmic flow in nerve cells. The cell line has been used for studies on the mechanism of vinblastine precipitation of microtubular protein, the kinetics of GTP binding to isolated protein, the turnover of microtubules in vivo, and the synthesis and assembly of microtubular protein. The World Organization for Animal Health (OIE) uses the cells for routine diagnosis of rabies.

The Transfection Reagent:

Neuro-2a Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Neuro-2a Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Neuro-2a Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of Neuro-2a Cell Avalanche™ Transfection Reagent for transfection on Neuro-2a Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into Neuro-2a Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NEURO-2A cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NEURO-2A cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NEURO-2A Cell Avalanche Transfection Reagent.

For Other Cells:

Neuro-2a Cell Avalanche™ Transfection Reagent (mouse neuroblastoma cell) can also be used on the following cells with high transfection efficiencies. IMR-32 Cell T98G Cell 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200