Cell Line Information:

Designations: NIH/3T3
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: embryo
Strain: NIH/Swiss
Cell Type: fibroblast
Applications: transfection host
Virus Susceptibility: Murine leukemia virus
Age: embryo
Comments: The NIH/3T3 is highly sensitive to sarcoma virus focus formation and leukemia virus propagation and has proven to be very useful in DNA transfection studies [PubMed ID: 222457].

The Transfection Reagent:

NIH/3T3 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on NIH/3T3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NIH/3T3 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of NIH/3T3 Cell Avalanche™ Transfection Reagent for transfection on NIH/3T3 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in NIH/3T3 Cells.
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Animal-free
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with serum
• Suitable for Reverse Transfection 
• Compatible with transfection in any plate formats
• Developed and manufactured by EZ Biosystems

 Data:FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH/3T3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH/3T3 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NIH/3T3 Cell Avalanche Transfection Reagent.FIG. 2. NIH/3T3 cells were transfected with GFP vector (pEGFP-N3) by using NIH/3T3 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells:NIH/3T3 Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.

3197-3 Cell
3T3-L1 Cell
BALB/3T3 clone A31
3T3-Swiss albino Cell
786-O Cell
Caki-1 Cell
Detroit 551 Cell
MG-63 Cell

 

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200