Cell Line Information:
Cell Line Name: NIH:OVCAR-3 (ATCC® HTB-161™) or OVCAR.3 or OVCAR-3
Organism: Homo sapiens, human
Tissue: ovary
Cell Type: epithelial
Culture Properties: adherent
Biosafety Level: 1
Disease; adenocarcinoma
Gender; Female
Ethnicity: Caucasian
Markers: androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive
Tumorigenic: Yes
Applications: This cell line is a suitable transfection host. It is an appropriate model system in which to study drug resistance in ovarian cancer. The presence of hormone receptors should be useful for the evaluation of hormonal therapy. Xenograft models have been used to show that treatment with 17 beta estradiol can induce progesterone receptors in this human ovarian carcinoma.
Comments: The NIH:NIH:OVCAR-3 line was established in 1982 by T.C. Hamilton, et al. from the malignant ascites of a patient with progressive adenocarcinoma of the ovary. Forms colonies in soft agar and has an abnormal karyotype. Resistant to clinically relevant concentrations of adriamycin, melphalan and cisplatin. Both cultured cells and xenografts exhibit androgen and estrogen receptors. NIH:OVCAR-3 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:
NIH:OVCAR-3 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NIH:OVCAR-3 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NIH:OVCAR-3 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in NIH:OVCAR-3 cells
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Free of animal-derived components
• Simplest and straightforward protocols to Increase experimental throughput
• Compatible with serum
• Suitable for Reverse Transfection  
• Compatible with transfection in any plate format
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Transfection Performance

• Transfection efficiency up to 90%
• Viability up to 95%

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH:OVCAR-3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH:OVCAR-3 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NIH:OVCAR-3 Cell Avalanche® Transfection Reagent.   FIG. 2. NIH:OVCAR-3 cells were transfected with GFP vector (pEGFP-N3) by using NIH:OVCAR-3 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

NIH:OVCAR-3 Cell Avalanche® Transfection Reagent (human ovary carcinoma cell) can also be used on the following cells with high transfection efficiencies. IGROV-1 Cell OVCAR-4 Cell OVCAR-5 Cell OVCAR-8 Cell SK-OV-3 Cell 293 Cell 293T/17 Cell Saos-2 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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