Cell Line Information:

Designations: NTERA-2 cl.D1 [NT2/D1]
Depositors: PW Andrews
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial-like, differentiation changes phenotype
Source: Organ: testis
Disease: malignant pluripotent embryonal carcinoma
Derived from metastatic site: lung
Applications: transfection host
Virus Resistance: UNTREATED CELLS: human cytomegalovirus (HCMV); human immunodeficiency virus (HIV-1, HTLV-III)
Age: 22 years
Gender: male
Ethnicity: Caucasian
Comments: The NTERA-2 cl.D1 cell line is a pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line. The parental NTERA-2 lines was established in 1980 from a nude mouse xenograft of the Tera-2 cell line. This clone differentiates along neuroectodermal lineages after exposure to retinoic acid (RA) or hexamethylene bisacetamide ((HMBA). The RA induced differentiation is characterized by glycolipid changes, appearance of neurons, and induction of homeobox (HOX) gene clusters. The cells exhibit high expression of N-myc oncogene activity.

The Transfection Reagent:

Transfection of pluripotent stem cells or stem-like cells, such as NTERA-2 cl.D1 cell line, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

NTERA-2 cl.D1 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on NTERA-2 cl.D1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NTERA-2 cl.D1 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of NTERA-2 cl.D1 Cell Avalanche™ Transfection Reagent for transfection on NTERA-2 cl.D1 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into NTERA-2 cl.D1 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NTERA-2 CL.D1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NTERA-2 CL.D1 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NTERA-2 CL.D1 Cell Avalanche Transfection Reagent.

For Other Cells:

NTERA-2 cl.D1 Cell Avalanche™ Transfection Reagent (human testis carcinoma cell) can also be used on the following cells with high transfection efficiencies. 786-O Cell Caki-1 Cell MDCK Cell Vero Cell DU 145 Cell 22Rv1 Cell VCaP Cell PC-3 Cell AR42J Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200