Cell Line Information:

Designations: PC-3
Organism: Homo sapiens (human)
Tissue: adenocarcinoma; prostate; metastatic site: bone
Age: 62 years
Tumor Stage: grade IV
Gender: male
Ethnicity: Caucasian
Morphology: epithelial
Growth properties: adherent
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells
AntigenExp: HLA A1, A9
Growth Properties: monolayer; the cells form clusters in soft agar and can be adapted to suspension growth
Depositors: M.E. Kaighn
The PC-3 was initiated from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62-year-old male Caucasian. The cells exhibit low acid phosphatase and testosterone-5-alpha reductase activities.

PC-3 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:

PC-3 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on PC-3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for PC-3 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of PC-3 Cell Avalanche™ Transfection Reagent for transfection on PC-3 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

References:

Akamatsu S, Wyatt AW, Lin D, Lysakowski S, Zhang F, et al. The Placental Gene PEG10 Promotes Progression of Neuroendocrine Prostate Cancer.Cell Rep. 2015 Aug 11;12(6):922-36. PubMed PMID: 26235627.

Features:

• Specifically optimized to deliver nucleic acids into PC-3 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with serum
• Suitable for Reverse Transfection 
• Compatible with transfection in any plate formats

DU 145 Cell 22Rv1 Cell VCaP Cell Vero Cell 293 Cell 293T/17 Cell Calu-3 Cell Caki-1 Cell MDCK Cell