Cell Line Information:

Designations: Ramos (RA 1)
Depositors: BC Giovanella
Isotype: IgM; lambda light chain
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Burkitt"s lymphoma (American)
Cell Type: B lymphocyte;
Cellular Products: immunoglobulin (surface and secreted) [1093] [22747].
Applications: transfection host
Receptors: interleukin 4 (IL-4); low affinity IgE (CD23) [23468]
Tumorigenic: Yes
Antigen Expression: CD23 [23468]
Age: 3 years
Gender: male
Ethnicity: Caucasian
Comments: The cells are negative for Epstein-Barr virus. They have about 1500 IL-4 binding sites per cell.
The cells are reported to secrete IgM (lambda light chain).

The Transfection Reagent:

Transfection of suspension cells, such as Ramos Cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments.

Ramos Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Ramos Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Ramos Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of Ramos Cell Avalanche™ Transfection Reagent for transfection on Ramos Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into Ramos Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems 

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