Cell Line Information

Designations: RAW 264.7
Depositors: WC Raschke
Growth Properties: adherent
Organism: Mus musculus
Morphology: monocyte/macrophage
Strain: BALB/c
Tissue: ascites
Disease: Abelson murine leukemia virus-induced tumor
Cell Type: macrophage; Abelson murine leukemia virus transformed
Cellular Products: lysozyme
Applications: Biological response; transfection host
Antigen Expression: H-2d
Age: adult
Gender: male
Comments: This line was established from a tumor induced by Abelson murine leukemia virus. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets. LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets.

The Transfection Reagent:

RAW 264.7 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on RAW 264.7 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for RAW 264.7 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of RAW 264.7 Cell Avalanche™ Transfection Reagent for transfection on RAW 264.7 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into RAW 264.7 Cells
• Highest efficiency to ensure experimental success
• Extremely gentle to cells 
• Simplest and straightforward protocols
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on RAW 264.7 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for RAW 264.7 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this RAW 264.7 Cell Avalanche Transfection Reagent.

For Other Cells:

RAW 264.7 Cell Avalanche™ Transfection Reagent (mouse macrophage tumor) can also be used on the following cells with high transfection efficiencies. J774A.1 Cell 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200